Supplemental Figure S1
-
Fig. S1. Spatial distribution of CTN, an HIV-TAT conjugated peptide, in PeA neurons. (A) The CTN peptide was constructed by fusion of the TAT protein-transduction domain with the C-terminus of NCS-1, which was previously found to be involved in P/Q-type Ca2+ channel facilitation (Tsujimoto et al., 2002). A tetra-glycine spacer was used to separate the two domains. (B) A BODIPY-conjugated CTN peptide (green) was constructed to follow the uptake of the peptide in PeA cells grown in CM. Cells were incubated for 5 minutes with the labeled peptide, fixed and then subjected to confocal imaging. CTN was distributed throughout the cytosol of cell somata, neurites and growth cones. (C) Confocal analysis of the distribution of CTN. CTN appeared uniformly distributed in the cytosol of somata, neurites and growth cones. The slice closest to the surface of the dish is indicated by 0 mm.
