|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. An NCS-1-related protein is expressed in L. stagnalis
neurons. (A) Protein sequence alignment of the full NCS-1 clone
from L. stagnalis (DQ099793) and the homologous proteins from A.
californica (Q16981), Rattus norvegicus (P62168), Drosophila
melanogaster (NP_996502) and Caenorhabditis elegans (P36608).
Alignment was performed in ClustalW and the plot made in GeneDoc. (B)
Western blot analysis of whole L. stagnalis and rat brains.
Polyclonal anti-rat NCS-1 antibody identified a single band migrating at
23 kDa in both rat and L. stagnalis ganglia preparations.
(C,D) Confocal imaging of anti-NCS-1 reactivity in a L.
stagnalis PeA neuron cultured for 3 days in CM. Epifluorescent image of
PeA cell stained with the anti-rat NCS-1 antibody show immunoreactivity
throughout the neuron (C). Analysis of cellular distribution of NCS-1
reactivity in the different regions of the cell (D, n=5 cells). The
data are presented as mean±s.e.m. Asterisk indicates a significant
difference (P<0.05) compared with that in the primary neurites.
Relativity intensities compared to primary neurites are 2.95±0.39 for
soma, 1.91±0.31 for branch points, 1.27±0.29 for secondary
neurites and 1.85±0.10 for growth cones.
|
