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Fig. 3. NCS-1 knockdown enhances neurite outgrowth in L.
stagnalis. (A) Phase-contrast images of a single PeA
neuron before and 24 hours after addition of water (right), 5 nM control
(middle) or NCS-1-specific (left) dsRNA. Cells were isolated and
cultured in CM for 24 hours before treatment. (B,C) Neurite
outgrowth was examined for the degree of branching (B) and the rate of
extension (C). Both the number of new neurites (the degree of branching) and
the rate of neurite extension were significantly higher after the
NCS-1-specific dsRNA treatment compared with controls. The number of
new neurites: mock control 1.50±0.39; control dsRNA (ctrl dsRNA)
1.04±0.39; and NCS-1 dsRNA 3.93±0.65. The rate of the
extension (µm/hour): mock control 3.77±0.67; control dsRNA
3.47±0.49; NCS-1 dsRNA 6.44±0.49. The data are
presented as mean±s.e.m. and obtained from 24 control, 43 control dsRNA
and 57 NCS-1-specific dsRNA-treated cells. Asterisk indicates
significant difference (P<0.05) from control condition.
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