(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. Sema3A overexpression in motoneurons disrupts nerve projections.
(A-F) Effects of control EGFP, Sema3AiresEGFP overexpression on spinal
nerve fasciculation (A,B), on motor projections (arrowhead, B,C) towards the
dermamyotome (outlined), the DRG (asterisk, D), and on dorsal ramus length (E,
double arrowhead). A shows NgCAM immunostaining on whole-mount HH24 embryos.
Immunolabeling (indicated in horizontal frames) was performed on transverse
cryostat sections (B,D,E) or on vibratome sections (C,F) and observed by
confocal microscopy. Scale bars: 100 µm. (G) Schematic of the
misrouting produced by Sema3A overexpression (defects, right side, in green;
normal pattern, left side).