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Figure 2


Fig. 2. Sema3A overexpression in motoneurons disrupts nerve projections. (A-F) Effects of control EGFP, Sema3AiresEGFP overexpression on spinal nerve fasciculation (A,B), on motor projections (arrowhead, B,C) towards the dermamyotome (outlined), the DRG (asterisk, D), and on dorsal ramus length (E, double arrowhead). A shows NgCAM immunostaining on whole-mount HH24 embryos. Immunolabeling (indicated in horizontal frames) was performed on transverse cryostat sections (B,D,E) or on vibratome sections (C,F) and observed by confocal microscopy. Scale bars: 100 µm. (G) Schematic of the misrouting produced by Sema3A overexpression (defects, right side, in green; normal pattern, left side).





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