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Fig. 6. Non cell-autonomous effects of motoneuronal Sema3A in conditions of
forced overexpression suggests that intrinsic Sema3A acts via secretion.
(A) Myc immunostaining on transverse sections of Sema3AiresEGFP HH25
embryo showing that Myc-tagged Sema3A is targeted to the defasciculated spinal
nerve (arrowhead). (B) Histogram showing the percentage (±s.d.)
of EGFP+ or EGFP- collapsed growth cones following
Sema3A and control supernatant exposure, of Sema3AiresEGFP, or control ventral
spinal cord explants. **, P<0.01; ***,
P<0.001 with Student's t-test; n.s.: non significant. The
total number of axons from three independent experiments is indicated on the
bars. (C,D) Histograms showing Nrp1 (NP1) and NgCAM
immunofluorescence intensities per unit of surface at the growth cone in
conditions of increased level of Sema3A overexpression. Immunolabeling of cell
surface Nrp1 and NgCAM was performed on non-permeabilized explants.
Fluorescence intensities were normalized to EGFP+ conditions.
**, P<0.01; ***, P<0.001 with
Mann-Whitney test. The number of axons examined is indicated on the bars.
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