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Figure 6


Fig. 6. Correlation between the level of GSH and the redox potential during development. (A) Relative amounts of NAD(P)H (grey bars), FAD++ (white bars) and GSH (black bars) in mature MII oocytes (M2 control), MII oocytes treated with 2 mmol/l BCNU for 1 hour (M2+ 2 mmol/l BCNU) and in MII oocytes treated with 2 mmol/l diamide for 15 minutes (M2+2 mmol/l diamide). The asterisks indicate that the values are significantly different from the control condition. (B) Relative amounts of NAD(P)H (grey bars), FAD++ (white bars) and GSH (black bars) in GV oocytes (fresh GV), in mature MII oocytes (fresh M2) and in two-cell embryos (2 cell). The asterisks indicate that the values are significantly different from the MII oocytes. P=0.019.





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