DEV02738 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Maternal E2f1 protein is present at the onset of zygotic RnrS expression. (A-D) Embryos were pulse labeled for 5 minutes with BrdU and stained for E2f1 (green), phospho-tyrosine (cyan) and BrdU incorporation (red). (A) Sibling control at G2₁₄. (B) E2f1⁷¹⁷²/E2f1⁷¹⁷² embryo at G2₁₄. (C) Sibling control at G2₁₄-S₁₅. (D) E2f1⁷¹⁷²/E2f1⁷¹⁷² embryo at G2₁₄-S₁₅. E2f1 mutants were identified by the reduction in E2f1 protein level. (E,F) Embryos were stained for E2f1 (green) and RnrS (red). (E) Sibling control. (F) E2f1⁷¹⁷²/E2f1⁷¹⁷² embryo. Scale bars: 50 μm.

• Supplemental Figure 2 -

  Fig. S2. RnrS expression declines during cycles 15 and 16. (A-C) w¹¹¹⁸ embryos were pulse-labeled with BrdU for 15 minutes and stained for BrdU incorporation (green). RnrS expression was detected by FISH (red). (A) Stage 9 embryo at early S₁₅. (B) Stage 9 embryo. The bracket denotes a region of late S₁₅ and the line indicates cells in early S₁₅. (C) Stage 10 embryo. The line denotes a region of early S₁₆. Scale bar: 50 μm.

• Supplemental Figure 3 -

  Fig. S3: E2f1 target gene expression persists inappropriately in dup mutants. (A-C) Stage 11 embryos were pulse-labeled with BrdU for 5 minutes, and stained for BrdU incorporation (green) and phospho-tyrosine (cyan). RnrS expression was detected by FISH (red). (A) w¹¹¹⁸. (B) dup^a1/dup^a1. (C) dup^a3/dup^a3. (D-G) Histochemical detection of RnrS expression by in situ hybridization of dup^a3 embryos (F) or embryos from dup^a1/+; UAS Rbf-280/+ females crossed to dup^a1/+; prd-Gal4/+ males (D, E and G). (D) Sibling control embryo. (E) Embryo with dup^a1/dup^a1 phenotype. (F) dup^a3/dup^a3 embryo. (G) dup^a1/dup^a1; UAS Rbf-280/prd-Gal4 embryo. UAS Rbf-280 expression suppresses ectopic RnrS caused by dup mutation (arrow). Scale bar in A-C: 50 μm.