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Fig. 2. E2f1-target gene expression is terminated in mutants containing ectopic
CycE-Cdk2. Embryos were pulse-labeled with BrdU for 5 minutes (A-D)
or 15 minutes (E) and were stained for BrdU incorporation (green) and
phospho-tyrosine to highlight cell boundaries (cyan). RnrS expression
was detected by FISH (red). (A) Stage 10 w1118 control
embryo. The bar denotes S16 in the dorsal epidermis and the bracket
marks cycle 15 in the ventral epidermis. (B) Stage 10
dap4454/dap4454 embryo. Note that
phospho-tyrosine staining was omitted because anti-ß-galactosidase was
used to distinguish CyO P[wg-lacZ]-containing embryos from the dap
mutants. (C) Control embryo that is a sibling of the embryo in D. The arrow in
the BrdU image denotes cells of the anterior spiracle primordium that normally
enter S17. (D) Stage 11
dap4454/dap4454 embryo. (E)
Df(1)biD3/Df(1)biD3 fzr mutant embryo. Scale bars: 50 µm.