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Figure 2


Fig. 2. E2f1-target gene expression is terminated in mutants containing ectopic CycE-Cdk2. Embryos were pulse-labeled with BrdU for 5 minutes (A-D) or 15 minutes (E) and were stained for BrdU incorporation (green) and phospho-tyrosine to highlight cell boundaries (cyan). RnrS expression was detected by FISH (red). (A) Stage 10 w1118 control embryo. The bar denotes S16 in the dorsal epidermis and the bracket marks cycle 15 in the ventral epidermis. (B) Stage 10 dap4454/dap4454 embryo. Note that phospho-tyrosine staining was omitted because anti-ß-galactosidase was used to distinguish CyO P[wg-lacZ]-containing embryos from the dap mutants. (C) Control embryo that is a sibling of the embryo in D. The arrow in the BrdU image denotes cells of the anterior spiracle primordium that normally enter S17. (D) Stage 11 dap4454/dap4454 embryo. (E) Df(1)biD3/Df(1)biD3 fzr mutant embryo. Scale bars: 50 µm.





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