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Fig. 6. The initial termination of E2f1-target gene expression does not require
Rbf1. Rbf114 maternal and zygotic null embryos were
pulse-labeled with BrdU for 15 minutes and stained for E2f1 (green) and BrdU
incorporation (cyan). RnrS expression was detected by FISH (red).
(A) Stage 10 embryo; bracket marks the dorsal epidermis in
S16 and the bar indicates cycle 15. (B) Stage 11 embryo; the
epidermal cells are in G216. (C) Stage 13 embryo; arrow
indicates epidermal cells arrested in G117 and the bracket denotes
epidermal cells inappropriately incorporating BrdU. Scale bar: 50 µm.