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Figure 4


Fig. 4. Rescued glucagon-producing cells have normal {alpha}-cell characteristics. (A-D) Immunofluorescence staining for glucagon (red) and ghrelin (green) at E12.5 and E18.5 on wild-type (A,C) and Nkx2.2-/-;Nkx2.2hdEnR (B,D) pancreata. There are a small number of glucagon-ghrelin co-staining cells (yellow) in wild-type and rescued pancreata (20x magnification). (E,F) Glucagon-positive cells (green) co-express the {alpha}-cell marker Pax6 (red) in wild-type (E) and rescued (F) islets at E18.5. (40x magnification.)





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