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Fig. 9. Marker analyses and fate mapping of tissues responding to HH signaling
in Shh mutants. (A-J) Tamoxifen-induced Cre labeling with
the same experimental conditions as those described in
Fig. 8. (A-D) E14.5 specimens
were co-stained for LacZ activity (green) and immunohistochemically stained
for differentiation markers (brown). (A,B) In the bladder (but not in the GT)
LacZ labeled mesenchyme co-stained with anti-smooth muscle myosin (SMM). (C,D)
The LacZ-positive dorsal GT mesenchyme overlapped with the tissue expressing
AR. (E-J) In Shh mutants, reduced LacZ staining was detected in the embryonic
GT and bladder mesenchyme (compare E with H, and F,G with I,J). (K) A
schematic depicting the distribution of HH responding tissues at E9.5-10.5 and
E13.5. b, bladder; bw, body wall; c, cloaca; cm, cloacal membrane (the ventral
side of the cloaca composed by inner endodermal epithelia with surface
ectoderm); gt, genital tubercle; hg, hindgut; pcm, peri-cloacal mesenchyme;
up, urethral plate.
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