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Figure 4


Fig. 4. Fate of Notch1-activated cells in the vasculature. (A) N1::cre activity marks the endothelial lining of the dorsal aorta (DA) but not of adjacent posterior cardinal vein (PCV) at E12.5. (B) By contrast, Notch1-deficient cells populate the PCV but do not contribute to the DA. (C,D) At E14.5, complete labeling of the endothelial lining of the aorta and intrasomitic arteries is observed (C, arrowhead) consistent with NICD presence as detected with {alpha}-VLLS staining (D, arrowhead) of endothelial cells. (E) Whole-mount X-Gal staining of yolk sac of E16.5 N1::cre embryo showing labeling of umbilical arteries, whereas maternal vasculature and veins are not labeled. (F) Complete lack of contribution from Notch1-deficient, R26 lacZ-marked cells to the yolk sac vasculature (arrow). A normal contribution is seen to the capillary plexus (arrowhead). (G,H) N1 activity in adult vasculature labels endothelial cells and a few smooth muscle cells (arrowhead) in the in the arteries(G) and veins(H). Magnification: A,B,C,F 20x; D,E,G,H, 40x





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