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Fig. 4. PAX77 cells are under-represented in the cortical proliferative layers
of
PAX77+;Tg-
wild-type;Tg+
chimeric embryos at E16.5. (A,B,F,G) Coronal sections through the cortex
of a PAX77+;Tg-wild-type;Tg+
chimera at E16.5. (A,B) Tg+ cells (marked with brown
dots) and Tg- cells were counted in the proliferative layers (VZ
and SVZ), intermediate zone (IZ) and cortical plate (CP). (C-E) Ratios
of observed/expected contributions of Tg- (i.e. PAX77) cells in (C)
VZ and SVZ, (D) IZ and (E) CP. (C) PAX77;Tg- cells are
significantly under-represented in the proliferative layers along the chimeric
cortex and (D) in the IZ of the rostral chimeric cortex. (F,G)
Example of Tg+ (arrow) and Tg- (arrowhead) apical
progenitors in M-phase labelled with anti-phosphorylated histone H3 (grey).
Scale bars: 50 µm in A; 10 µm in B,F,G.
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