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Fig. 1. Localization of Scmh1 in the adult testes and spermatocytes.
(Aa) In situ hybridization using antisense probe. Stages of
seminiferous tubules are given. (Ab) Higher magnification view of
seminiferous tubule at stage VII shown in a. Arrows and arrowheads indicate
pachytene spermatocytes and round spermatids, respectively. (Ac)
Control slides using sense probe. (Ba) Immunohistochemical localization
of Scmh1 of wild-type testes. (Bb) Higher magnification view of
seminiferous tubule shown in a. Arrows and arrowheads indicate pachytene
spermatocytes and round spermatids, respectively. (C)
Immunocytochemical detection of Scmh1 gene products from zygotene to pachytene
stage spermatocytes, which were prepared from day 18 pp wild-type testes.
(Ca-Cd) Spermatocyte spreads were substaged into early (a) and late (b)
zygotene and early (c) and late (d) pachytene stages based on anti-Scp3 (red)
immunostaining and morphology. Scmh1 (green) was localized in the nuclei at
each stage, but was mostly excluded from the X and Y chromosome territory at
late pachytene stage, as indicated by dotted lines. (Ce) Reciprocal
subnuclear localization of Scmh1 and 
H2A.X indicated exclusion of Scmh1
from the XY body. The XY body is indicated by dotted lines. (Cf)
Reciprocal subnuclear localization of Scmh1 and dimethylated H3-K9 indicated
exclusion of Scmh1 from the XY body. The XY body is indicated by dotted
lines.
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