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Fig. 2. Expression pattern of other PcG proteins and trimethylated H3-K27 at
pachytene stage spermatocytes. (A) Immunocytochemical detection of
Prc1 components in pachytene spermatocytes. (Aa,Ab) Phc1 (a) and
Phc2 (b) were excluded from the XY body demarcated by extensive accumulation
of 
H2A.X. (Ac-Ae) Bmi1 (c), Rnf110 (d) and Cbx2 (e) were
excluded from the XY body demarcated by extensive accumulation of uH2A. The XY
body is indicated by dotted lines. (Af) Reciprocal subnuclear
localization of Phc2 and dimethylated H3-K9 indicated exclusion of Scmh1 from
the XY body. (B) Immunocytochemical detection of trimethylated H3-K27
from late zygotene to pachytene stage spermatocytes. Spermatocytes were
immunostained by using anti-trimethylated H3-K27 (red) and anti-SCP3 (green).
The X and Y chromosome territory is indicated by dotted lines.
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