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Fig. 3. Testicular abnormalities in Scmh1-/- mice.
(A) Cross-sections of testes from day 35 pp wild-type (Aa) and
Scmh1-/- (Ab) mice. Sections were stained with
Hematoxylin and Eosin (HE). (B) The frequency of
Scmh1-/- mice in which seminiferous tubules were
morphologically affected during first-wave spermatogenesis. Days after birth
are shown. At each age, more than ten mutants were examined. Mutants over 8
weeks of age were collected and indicated as adults (Ad). (C) Increased
apoptotic spermatocytes in Scmh1-/- testes. (Ca-Cc)
Incidence of apoptosis in wild-type testes at day 7, 15 and 19 pp.
(Cd-Cf) Incidence of apoptosis in Scmh1-/- testes
at day 7, 15 and 19 pp. (Cg,Ch) Higher magnification views of
individual seminiferous tubules shown in e and f. Outline of seminiferous
tubules are indicated by dotted lines. (D) The expression of
stage-specific markers during spermatogenesis in wild-type and unaffected and
affected Scmh1-/- testes at day 35 pp, as revealed by
semi-quantitative RT-PCR. ß-actin was used as a standard to
verify the equal amounts of cDNA. Primers used in each reaction are shown in
Table 1. Scale bars: 100 µm
in A,B,Ca-Cf; 10 µm in Cg,Ch.
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