|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Expression of N and its ligands in the VNC. (A-F) Stage 15
embryo (genotype repo-Gal4;UAS-mCD8GFP) co-labeled for N (red), GFP
(blue) and HRP (green). (A) N is found on most neurons and is highly expressed
in the neuropil. (B,C) Single optical section dorsal to the neuropil reveals N
expression (red) along the perimeter of many LG marked by the
membrane-targeted GFP. (B',C') In a cross-section through LG and
the longitudinal connectives, N co-localizes with the membrane of LG
(arrowheads) and channel glia (arrow). (D,E) N expression overlaps with
anti-HRP in the dorsal neuropil (arrowheads in E), while HRP is excluded from
glia (F). The dotted line in B indicates the anteroposterior (a-p) level of
the cross-section in B',C', and D-F. The dotted line in B'
indicates the dorsoventral (d-v) level of section for B and C. (G) Dl
expression in a cluster of cells (arrow) near the lateral edge of the VNC of a
stage 15-16 embryo. (H) Dl is expressed on proximal axons (arrowheads
in G,H) although it is largely excluded from the distal axons in the region of
the longitudinal connectives. (I) Ser is expressed on the cell bodies
(arrowheads) and axons of two or three neurons in each hemisegment. Scale bar:
10 µm. (J) Cartoon depicting a summary of the expression of N, Dl
and Ser relative to expression of Pros in the anterior LG.
|
