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Figure 8


Fig. 8. Fgf3 or Fgf8 is sufficient to induce foxi1-positive EB precursors and phox2b-positive EB neurons in fgf3+8 morphants. (A,C) Dorsal views; (B) lateral views. (A,B) Zebrafish embryos were injected with hs-fgf3myc or co-injected with hs-fgf8 and hs-gfp plasmids at one-cell stage, heat shocked at 10-13 hpf and then assayed for foxi1or phox2b expression (blue) and Myc or GFP expression (red and green, respectively). Fgf3myc or Fgf8 efficiently rescued foxi1 (brackets in A) or phox2b (arrows in B) expression in fgf3+8 morphants. Note the difference between foxi1 expression domain adjacent to Myc or GPF-expressing cells and contralateral side. (C) fgf3+8 morphant





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