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Fig. 7. gcm and gcm2 are expressed in central brain neuronal
lineages. (A-A'') gcm (A) and gcm2
(A') double in situ hybridization on wild-type Drosophila LIII
brain hemispheres. (A'') Merge of gcm (red) and gcm2
(green) labeling. gcm and gcm2 RNAs are coexpressed in
lamina neuronal [lamina precursor cells (LPCs)] and glial [glial precursor
cells (GPCs)] progenitors as well as in a central brain cluster (area
encircled by dotted line in A-A''). (B-C') GFP
immunolabeling on gcm-gal4,UAS-mCD8GFP (gcm>GFP) LIII (B)
or whole-mount adult brains (C,C'). Note that the GFP expression profile
mimics gcm expression pattern in larvae. Anterior (C) and posterior
(C') views of the same adult brain are shown. Neuronal somata of
dorsolateral (dcbcs) and medial (mcbcs) clusters are encircled by white (C)
and yellow (C') dotted lines, respectively. (D-D''') GFP
(green), phospho-Histone H3 (red) and DAPI (blue) colabeling at late LI
gcm-gal4,UAS-ncGFP larva. (D''') Merge of D-D''; note that
a single GFP-positive cell undergoes mitosis as shown by phospho-Histone H3
expression. Scale bars: 40 µm in A-A'';
40 µm in B; 50 µm in C-C'';400 µm in D-D''.
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