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Fig. 9. Fly, chick and mouse gcm genes induce expression of neuronal
and glial markers in HeLa cells. GFAP
(A,B,C,D), GFP
(A',B',C',D'), Tuj1
(A'',B'',C'',D'') and DAPI
(A''',B''',C''',D''')
colabeling and merges
(A'''',B'''',C'''',D'''')
of HeLa cells transfected with expression vectors carrying c-Gcm1
(A-A''''), gcm (d-gcm1 in B-B''''),
mouse Gcm1 (m-gcm1 in C-C'''') or vector as
control (D-D''''). Scale bar: 20 µm. (E) Percentage of
transfected cells expressing neuronal/glial markers. n indicates the
number of GFP-positive cells counted. Note that all gcm1 genes induce
the expression of GFAP and Tuj1 markers and that these two markers colocalize
in most transfected cells.
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