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Fig. 1. Expression patterns, mutant phenotypes and genomic organization of slp. (A) Adult eye of the slp2 insertion line (slp2^AVm) shows pigmentation in the anterior/ventral domain of the eye. (B) Genomic map of the slp locus showing the site of insertion of slp2^AVm and the deletion induced (slp^S37A, dotted lines). (C) slp-lacZ expression in the second-instar eye disc. (D) mirr-lacZ expression in the second instar. (E) Double staining of slp-lacZ (red) and Mirr (green) of second-instar eye disc. The two domains abut in the center of the disc. (F) Double staining of slp-lacZ (red) and Mirr (green) of third-instar head disc. The white arrowhead indicates the gap between the slp and Mirr expression domains. Arrows indicate slp expression in the dorsal regions of the extreme anterior of the eye disc, and in the posterior dorsal (relative to the eye) regions of the antennal disc. (G-I) Survival of slp^S37A mutant clones (black) in third-instar eye discs, induced at different times of development. Yellow arrowhead indicates morphogenetic furrow (MF). (G) When clones are induced 0-24 hours AEL, twin spots (arrow) are readily evident in the ventral regions, but mutant clones are rarely observed. (H) Clones induced 24-48 hours AEL do not survive ventrally ahead of the MF (arrow points to a twin spot), but can be observed behind (white arrowheads), but are rounded with smooth borders. (I) Clones induced 48-72 hours AEL. slp^S37A mutant clones (white arrowheads) survive ahead of the MF but show smooth borders. In all images, dorsal is up and posterior is left.

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