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Figure 1


Fig. 1. Characterization of the enhancer that drives LIN-3 expression in the pre-AC/pre-VU and AC. (A) In wild-type animals, Z1.ppa and Z4.aap cells always become VU cells and Z1.ppp and Z4.aaa cells have equal potential to become the AC or a VU. (B-D) In wild-type animals, LIN-3::GFP is expressed (B) in the pre-AC/pre-VU cells before AC/VU specification and (C) in the AC after the specification. Multiple gonadal cells express LIN-3::GFP after specification in the lin-12 (null) background (D). LIN-3::GFP is not expressed in any gonadal cells of the lin-12 (gain-of-function) animals after mid-L2 stage where all four pre-AC/pre-VU cells are transformed into VU cells (data not shown). (E) Anchor Cell-specific Enhancer of LIN-3 (ACEL) is sufficient to express GFP in both AC and pre-AC/pre-VU cells. Site-directed mutations of the E-boxes or FtzF1 NHR binding site, but not those in other regions, eliminate GFP expression in both cell types. Expression of these constructs in the AC was reported previously (Hwang and Sternberg, 2004).





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