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Fig. 6. HLH-2 and LAG-1 bind to egl-43 regulatory regions.
(A,B) EMSA with in vitro translated (TNT) LAG-1. Shifted
complexes (B1 and B2) appear in the presence of LAG-1. The binding complexes
are competed by DNAs containing the wild-type (w) but not the mutated (m)
LAG-1 binding sites. `F' indicates the migration of the free DNA probe.
(C,D) EMSA with purified HLH-2. A shifted complex `B' appears
with HLH-2 and the wild-type ACEL-like probe, but not with Luciferase or with
the probe on which both E-boxes are mutated. (D) The binding complex is
competed by DNAs containing the wild-type (w) but not the mutated (m) E-boxes.
DNA sequences are listed in Table
1; the numbers in B and D correspond to those of DNA competitors
in Table 1. (E) PCR
analysis from ChIPs performed with the extracts from animals expressing
SEL-8::GFP. Pre-IP represents the input extracts subjected to IP. The
5'-region of lip-1, which contains four LAG-1 binding sites and
was shown to be immunoprecipitated with LAG-3 (SEL-8) antibodies
(Lee et al., 2006), was used
as a positive control.
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