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Figure 4


Fig. 4. LIN-12::GFP and LET-23 localization. (A,B) Schematic depiction of the localization of an apical or a basolateral protein in the VPCs relative to the adherens junction (AJ) in a lateral (A) or a ventral view (B). (C) Lateral and (D) ventral views of the distribution of LIN-12::GFP expressed from arIs41 (green) relative to the AJ (red). (E) Lateral and (F) ventral views of LIN-12::GFP (green) and LET-23 (red). (G) Lateral view of LIN-12::GFP distribution (green) in rme-6(b1018) relative to the AJ (red). (H) Quantification of LIN-12::GFP fluorescence intensity in sel-2(+) and sel-2(-) hermaphrodites (see Materials and methods). In photomicrographs, `gon' marks LIN-12::GFP staining in the gonad, which is close to the basolateral surface of the VPCs, and arrows indicate basolateral LIN-12::GFP. P5.px, P6.px and P7.px are indicated.





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