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Fig. 2. hts antibodies and tagged proteins show differential localization of Hts proteins. (A-D) Germaria and (E-H) stage 9 egg chambers labeled with the designated antibody. (A) Regions of the germaria are underlined (King, 1970). Region 1 (R1), all dividing cells; Region 2, 16-cell cysts not surrounded by follicle cells (R2a, cysts are spherical; R2b cysts are lens shaped and span the width of the germarium); Region 3 (R3), cysts surrounded by follicle cells. (A,B) htsF and 1B1 antibodies label fusomes in the germline (arrow) and follicle cell membranes (arrowhead). (C) htsM antibody labels only follicle cell membranes. (D) htsRC antibody labels puncta and then RCs starting in Region 2a. (E-G) htsF, 1B1 and htsM antibodies label follicle cell membranes exclusively; there is no localization in the germline. (H) htsRC antibody labels only RCs. (I-K) Transgene expression in germaria co-stained with 1B1 (red). (I) ShAdd::Ven localizes to the fusome until Region 3 and then is present at a very low level in the cytoplasm. (J) Cer::Ovhts localizes to the fusome in the germaria based on staining with anti-GFP antibodies. (K) Ovhts::GFP starts as puncta in Region 2 and then localizes to RCs throughout oogenesis. (L,L') Higher magnification of a germaria expressing Ovhts::GFP. Puncta appear in Region 2a (arrowhead) near the fusome and start to become RCs (arrow) in Region 2b. Scale bars: A-D,I-L,10 µm; E-H, 20 µm.

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