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Figure 4


Fig. 4. Expression of transgenes can rescue RCs but not the fusome. (A-F) Germaria of hts{Delta}G mutants expressing the designated hts transgenes. (A,B) Expression of Cer::Ovhts results in no fusome rescue by either anti-GFP antibody (A, green) or htsF (B, red) staining. However, there is rescue of Ovhts-RC localization to the RC shown by staining with htsRC antibodies (B, green). (C) Expression of Ovhts::GFP results in no fusome rescue (1B1 staining, red) but does rescue Ovhts-RC::GFP to RCs (green). (D) Expression of ShAdd::Ven results in no fusome rescue (1B1 staining, red). ShAdd::Ven (green) is cytoplasmic. (E,F) Expression of ShAdd::Ven (green) with either Cer::Ovhts (E) or Ovhts::GFP (F, green) does not rescue the fusome (E and F, green) or fusome staining with 1B1 (F, red), but does rescue RCs as seen with either transgenic GFP (F, green) or staining with htsRC antibody (E, red). Scale bars: 10 µm.





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