|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Continuous Ovhts expression is necessary for Ovhts-RC and actin
localization to RCs. (A) Expression of P{UASH-Ovhts::GFP}
with MTD-Gal4 results in expression of Ovhts::GFP during all stages
of oogenesis and continuous localization to RCs. (B) Expression of
P{UASH-Ovhts-GFP} with the P{bam-GAL4} driver in wild-type
ovaries shows expression and localization of Ovhts::GFP to RCs in the germaria
and faintly in stage 2 (arrowhead). There is no Ovhts::GFP protein on RCs at
later stages that are actinpositive (arrow). (C-D) Expression of
P{UASH-Ovhts::GFP} in
hts
G with P{nos-Gal4}. The
P{nos-Gal4} driver produces Ovhts::GFP expression (green) in early and
late stages, but no expression in intermediate stages. Staining with
Rhodamine-conjugated phalloidin shows that F-actin localization to RCs mirrors
Ovhts::GFP expression. (D) Higher magnification of an intermediate stage egg
chamber that is not expressing Ovhts::GFP. F-actin is in clumps that may be
degenerating RCs. Scale bars: A,B, 20 µm; C,D, 10 µm.
|
