Distinct functions of -Spectrin and ß-Spectrin during axonal pathfinding

DEV02758 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Spectrin expression in the embryonic nervous system. Single confocal sections of dissected nervous systems of wild-type stage-16 embryos stained for expression of β-Spectrin (A,C-D′ white/red). Wrapper expression labels the midline glial cells (C′, green). The nuclei of the lateral glial cells were labeled using anti-Repo antibodies (D′, green). (A) β-Spectrin is expressed by all CNS neurons. Reduced levels are found in the longitudinal connectives. (B) α-Spectrin is expressed in a very similar pattern. (C,C′) Elevated expression of β-Spectrin is found at the CNS midline (arrowhead), overlapping with the midline glia marker Wrapper (C′, arrowhead). (D,D′) All lateral glial cells are labeled by Repo expression and express β-Spectrin at similar levels as the cortical neurons.

• Supplemental Figure 2 -

  Fig. S2. Expression of α-Spectrin. Wild type and different mutants were kept at the temperature indicated. Protein extracts were prepared and tested for α-Spectrin expression. The stability of neither the wild-type nor a mutant α-Spectrin protein is dependent on temperature.