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Figure 4


Fig. 4. Non-autonomous effects of ß-spectrin on axonal patterning. Dissected preparations of stage-16 embryos stained for HRP (green) and ß-Galactosidase or ß-Spectrin, as indicated. (A,A') The ap::Gal4 driver directs {tau}lacZ expression into a small subset of ipsilateral-projecting neurons. (A') Notice the distance between the ß-Galactosidase-expressing fascicles (yellow bar). (B,B') Following expression of ß-spectrin in the ap::Gal4 pattern in kus mutants, the axonal trajectories are found at the same distance from the CNS midline as in hemizygous-mutant kus-mutant embryos (C,C'). (D,D') The sim::Gal4 driver directs {tau}lacZ expression into the MP1 neurons and some midline cells. Notice the distance of MP1 fascicles to the midline (yellow bar). (E,E') Following expression of ß-spectrin in the sim::Gal4 pattern in kus mutants, the MP1 axons are found at the same distance from the CNS midline as in hemizygous kus-mutant embryos (F,F').





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