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Fig. 1. Disruption of the Fgfr2 alleles in prostate epithelium. (A) Schematic of the floxed Fgfr2 alleles for conditional disruption. The genomic DNA containing exons 6-10 and the adjacent introns is shown. The primers for PCR genotyping and FGFR2 expression analyses are indicated. (B) The Fgfr2^cn alleles only encode a truncated ectodomain. (C) PCR genotyping. Genomic DNAs extracted from different lobes of Fgfr2^cn and control prostates of 3-week-old mice were PCR analyzed with the indicated primers. Primers f1 and f2 amplify a fragment of 207 bp from floxed Fgfr2 alleles. Primers f1 and f3 amplify a fragment of 471 bp from Fgfr2-null alleles, and give no amplification for wild-type Fgfr2 or Fgfr2^flox alleles. (D,E) Diminished FGFR2 expression in the epithelium of Fgfr2^cn prostates. The expression of FGFR2 was assessed with RT-PCR (D) and in situ hybridization (E). Primers R2f and R2r amplify both FGFR2IIIb (IIIb) and FGFR2IIIc (IIIc) isoforms; primers b and t only amplify FGFR2IIIb isoform, primers c and t only amplify FGFR2IIIc isoform. -, negative control without cDNA templates. Panel E shows strong expression of FGFR2 in the epithelial compartment of control prostates, which was diminished in Fgfr2^cn prostates. ap, anterior prostate; dlp, dorsolateral prostate; vp, ventral prostate; S, signal peptide; I/II/III, immunoglobulin loop I, II and III, respectively; TM, transmembrane domain; F/F, homozygous Fgfr2^flox mice; CN, Fgfr2^cn mice.

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