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Fig. 5. Ectopic activation of the canonical Wnt pathway induces zic gene
transcription. (A-C) Embryos were injected with
hs:ßCat-gfp DNA, heat shocked at shield-stage, and
assayed for zic2a (purple) and gfp (orange) at
late-gastrula. (A) ßCat-gfp is expressed in the medial neural
plate; the zic2a pattern is normal (no ectopic expression;
n=10, two experiments). (B,C) ßCat-gfp is expressed in
lateral ectoderm; zic2a is ectopically expressed (arrows;
n=8, 8 ectopic, two experiments). (D) Control embryo
expressing hs:gfp in lateral ectoderm showing no ectopic
zic2a expression (n=18, 0 ectopic, one experiment). a,
anterior. (E-G) Zic2aD5:gfp embryos injected with
GR-Lef RNA and assayed for gfp RNA at late-gastrula
(purple). (E) GR-Lef-injected embryo showing weak ectopic gfp
(arrow). (F,G) GR-Lef-injected embryos treated with DEX (F) or DEX and CHX
(G). (H) Uninjected zic2aD5:gfp control treated with DEX.
(I) Summary of GR-Lef overexpression experiments, showing the
proportion of embryos expressing gfp for each treatment condition.
The number of embryos scored for each condition is listed along the
x-axis.
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