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Figure 6


Fig. 6. Multiple Tcf/Lefs are required for zic gene expression. Embryos injected with the MOs shown at the lower left of each panel and stained for zic2aD5:gfp (A,C,E,G,I) or zic2a (B,D,F,H,J) at 18 hpf. (C-D) Strongly affected tcf3 morphants had anterior trunctions and reduced zic2aD5:gfp (asterisks) and zic2a domains. (E-J) lef1 morphants showed a strong reduction in zic2aD5:gfp (E) and a mild reduction in zic2a (F). tcf7 morphants showed a mild reduction in zic2aD5:gfp (G), but normal zic2a (H). lef1+tcf7 morphants showed a strong reduction in zic2aD5:gfp (I) and mild reduction in zic2a (J). All views are lateral, anterior to the left. Arrowheads indicate the midbrain.





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