DEV02777 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Expression analyses of Hesr1 and Hesr2 in E9.5 mouse embryos. Section in situ hybridization analysis of the expression of Hesr1 (A,B) and Hesr2 (C,D) in wild-type (A,C), Hesr1-ME (B) and Hesr2-ME (D) hearts. (A) Hesr1 is detected in a normal pattern in the atrial myocardium. (B) In the Hesr1-ME heart, Hesr1 is detectable in not only the atrial myocardium but also in both the compact and trabecular zone of the ventricular myocardium. (C) Hesr2 is detectable in a normal pattern in the compact zone of the ventricular myocardium in the wild-type embryonic heart. (D) In the Hesr2-ME heart, Hesr2 expression is also evident in the atrium, as well as in the trabecular zone of the ventricles. LA, left atrium; LV, left ventricle; RA, right atrium; RV, right ventricle.

• Supplemental Figure 2 -

  Fig. S2. The expression of early myocardial markers is unaffected in Hesr1- or Hesr2-misexpressing mouse heart at E9.5. Chamber identities were examined by whole-mount in situ hybridization. Markers for the ventricular chamber, Cited1 (A-C), and for demarcation of both the atrial and ventricular chambers, Anf (D-F) and Chisel (G-I), were used. Boundaries between the chambers and AV canal are less evident in the Hesr1-ME heart (B,E,H,) and appear to be lost in the Hesr2-ME heart (C,F,I), compared with the wild-type embryo (A,D,G). (J-L) Tbx20 expression, which is an early marker for the heart and which is known to be an essential factor for Hesr1 and Hesr2 expression, is unaffected in the Hesr1-ME or Hesr2-ME heart. The arrowheads in D-F,H,I indicate the AV boundaries for the atrium and/or ventricle. LA, left atrium; LV, left ventricle.