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Fig. 1. The possible involvement of Hesr1 and Hesr2 in AV boundary
formation. (A-F) Expression patterns of Hesr1 and
Hesr2 in wild-type mouse embryos at E9.5 revealed by whole-mount in
situ hybridization. The specific expression of Hesr1 in the atria
(A,D) and of Hesr2 in the ventricles (B,E) can be seen, but neither
gene is found to be expressed in the AV canal (AVC) (C,F). The heart regions
in A-C are magnified in D-F, respectively. (G-J,L-S) The
expression patterns of AV boundary markers in wild-type (G,L,P),
Hesr1-null (H,M,Q), Hesr2-null (I,N,R) and
Hesr1-Hesr2 double-KO (J,O,S) mouse embryonic hearts at E9.5. To
demarcate the boundaries between both the atrium and ventricular chambers and
the AV canal, the AV myocardial markers Bmp2 (G-J) and Tbx2
(P-S), and the chamber marker Anf (L-O), were used as probes.
(K) The ratio of the AV canal area was calculated by determining the
Bmp2 expression area in comparison with the entire heart area from a
lateral side view (n=4, P<0.005, t-test). The
brackets in G-J and L-P indicate the AV canal. The arrowheads in Q-S indicate
the affected AV boundaries. The gene probes used (upper right) and genotypes
examined (lower right) are indicated in each panel. LA, left atrium; LV, left
ventricle.
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