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Fig. 2. Misexpression of Hesr1 and Hesr2 in the entire
embryonic mouse heart at E9.5. (A-E) The expression of
Hesr1 (A,E) and Hesr2 (B,D) was examined by whole-mount in
situ hybridization of double-transgenic mouse embryos. The embryos were
produced by crossings between Mesp1-Cre and either
CAG-lox-CAT-lox-Hesr1 (A,D) or
CAG-lox-CAT-lox-Hesr2 (B,E) mice. (C) Expression
levels of Hesr1 and Hesr2 in two independent lines for each
gene derived from these misexpressing mouse embryonic hearts (n=10).
Both Hesr1 and Hesr2 are strongly induced (2.5-5.5 fold)
compared with the wild-type heart. (F-H) Sections stained with
Hematoxylin and Eosin showing a normal morphology for the atrium, AV canal
(bracket), and the ventricle in the wild-type embryonic heart (F). The width
of the AV canal (bracket) is smaller in the Hesr1-ME heart (G). The
atrium and ventricle seem to be directly connected (arrowhead) in the
Hesr2-ME heart (H). (I-K) Analysis of cardiomyocytes in the
left ventricles of the mouse embryonic heart by transmission electron
microscopy. Myofibrils were observed to be thin and disorganized in the
Hesr1-ME (J) and Hesr2-ME (K) hearts, compared with wild
type (I). In the Hesr2-ME hearts, Z bands (arrowhead) were sometimes
barely discernible (asterisks). Scale bars: 100 µm in F-H; 1 µm in I-K.
RA, right atrium; RV, right ventricle; LA, left atrium; LV, left
ventricle.
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