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Figure 5


Fig. 5. Expression of RhoA-ROCK and JNK signalling components during mouse neurulation. (A) RT-PCR analysis of RhoA, ROCK1, ROCK2, LIMK1 and LIMK2. Lane 1 contains molecular size standards: 800, 600 and 400 bp (top to bottom). Lanes 2-4 show expression in whole embryo homogenates at E7.5, E8.5 and E9.5, respectively. Lane 5 is no RT control. (B-J) Whole-mount in situ hybridisation of wild-type embryos at E8 (B-E; pre-somite headfold stage) and at E8.5 (F-J; 5-7 somites). PCP genes Vangl2 (B) and Daam1 (C), and the downstream signalling molecules RhoA (D), ROCK2 (E) are expressed throughout the late gastrulation E8 embryo. By E8.5, expression of Vangl2 (F) and Daam1 (G) becomes restricted to the hindbrain and upper spinal region, where neural tube closure is initiated (between double arrows in F-J). RhoA (H), ROCK2 (I) and JNK1 (J) are expressed in overlapping domains with the PCP genes at E8.5. Scale bars: in E, 0.2 mm for B-E; in J, 0.2 mm for F-J.





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