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Fig. 4. The segmentation process in Notch2-deficient nephron (N2) is impaired. (A,B) Cadherin-6-expressing cells are adjacent to E-cadherin-expressing cells in wild type (A), but no cadherin-6-expressing cells are detectable in mutant (B). (A'-B''') Serial sections stained with the segmentation markers Wt1, Pax2 and E-cadherin. Circles and arrowheads locate the same cells in adjacent sections to indicate similar structure across all sections. (C,D) Lim1 expression pattern in the renal vesicle is similar in wild-type and Notch2 mutant kidneys (white arrows). The cells at the very proximal end of the mutant nephron express high levels of Lim1 (D). This distribution is different from that in a wild-type S-shaped body (C). (E) In wild type, few Jag1-expressing cells are seen in the renal vesicle (arrowheads), but the population expands with the formation of the S-shaped body (circle). (F) A small cluster of Jag1-expressing cells is seen in each of the mutant nephrons. (G,H) Dll-1^LacZ and N1-ICD (arrowhead) are detectable in the renal vesicle in wild type. (I) In some cases, Jag1 is not co-localized with N1-ICD (arrowhead). N1-ICD remains detectable in Notch2-deficient nephrons (arrowhead, inset in I). Scale bars: 0.1 mm in B for A-B''' and in F for C-F; 0.05 mm in G-I.

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