spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Notch1-deficient ES cells contribute to various parts of the nephron. Kidneys were harvested from E16.5 embryos (A-F) or adults (G) and subjected to whole mount ß-gal staining. (A,C,E) Wild-type ES cells in E16.5 kidneys are found in early nephrons (arrowheads in A), PCTs (circled in C) and glomerular podocytes (arrowhead in E). (B,D,F,G) Notch1-/-; Rosa26 ES cells contribute to renal vesicles (arrows in B), S-shaped bodies (arrowhead in B) and podocytes in the capillary-loop stage (star in B). (D,D') Notch1-deficient ES cells (blue) contribute to LTL-labeled PCT together with wild-type cells (unstained). One LTL-labeled cross-section composed entirely from Notch1-deficient cells is shown in D (arrowhead). (F) Glomerular podocytes develop in the absence of Notch1 (arrowhead). The circle indicates the capillary lumen with red blood cells in it. (G) The adult chimeric kidney is stained with LTL (brown). Some of the LTL-labeled tubules in the inner cortex are entirely derived from Notch1-/- cells (black arrow). White and black arrowheads indicate a juxtamedullary glomerulus and a cortical glomerulus, respectively.





Right arrow Return to article