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Fig. 7. Notch1-deficient metanephroi are phenotypically wild type. Genotypes are marked above all panels. Where indicated, floxed alleles were recombined with the Pax2-Cre^tg/+ strain. (A,B,E,F) Whole-mount staining of LTL (green) and Ck8 (red) detects extensive renal tubulogenesis. Loss of Notch1 did not alter proximal tubule formation (B), whereas loss of Rbp-J resulted in loss of LTL-positive epithelial cells (F). Note that loss of Notch1 (see Notch1 staining in inset in C,D) or of Rbp-J in the duct did not prevent duct branching. (C,D,G,H) Glomerular podocytes are labeled with Wt1 (green). In G,H, whole-mount preparation is also stained with Ck8 (red). Wt1^High, synaptopodin-positive (red in C,D, inset in G) glomeruli are present in wild type (C), Notch1-deficient metanephroi (D) and Rbp-J heterozygotes (G), but not in Rbp-J deficient metanephroi (inset in H, no Wt1 or synaptopodin). Scale bars: 0.5 mm in B for A,B; in D for C,D; in F for E-F.

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