DEV02789 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Progressive nature of the muscle fibre defect in cho homozygotes. Slow muscle fibre arrangements and integrity as revealed by labelling with slow MyHc antibody (green). Left frame is a DIC image showing melanophore positions. The right frame combines this DIC image with a fluorescent image revealing F59 antibody labelling in one focal plane. Anterior trunk (A-F) and posterior trunk (G-L) are shown for WT (A,C,E,G,I,K) and cho mutant (B,D,F,H,J,L) embryos at 38 (A,B,G,H), 45 (C,D,I,J) and 67 hpf (E,F,K,L). Scale bars: 125 μm.

• Movie 1 -

  Movie 1. Timelapse of anterior trunk of WT embryo, beginning at 48 hpf. Anterior is to right, dorsal up.

• Movie 2 -

  Movie 2. Timelapse of anterior trunk of cho mutant, beginning at 48 hpf. Anterior is to right, dorsal up.

• Movie 3 -

  Movie 3. Confocal 3D reconstruction of slow muscle fibres in anterior trunk of 55 hpf WT, as revealed by slow MyHc antibody.

• Movie 4 -

  Movie 4. Confocal 3D reconstruction of slow muscle fibres in anterior trunk of 55 hpf cho mutant, as revealed by slow MyHc antibody.

• Movie 5 -

  Movie 5. Confocal 3D reconstruction of slow muscle fibres in anterior trunk of 30 hpf cho mutant, as revealed by slow MyHc antibody.