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Fig. 1. Timecourse of anterior trunk pigment pattern formation in WT and
cho mutants during period of collar formation. Lateral views of
WT (A,C,E) and cho mutant (B,D,F) embryos are shown.
Melanophores were seen on the lateral pathway in cho mutants and WT
siblings at 34 hpf (A,B), and were thereafter absent from WT, but accumulated
steadily in cho mutants (C-F). Note too the change in collar
melanophore morphology between 58 hpf, when their stellate morphology
correlated with migratory activity, and 74 hpf, when their more flattened
shape reflects a more static phase. Scale bar: 50 µm. (G) Total
number (means±s.e.) of melanophores on lateral pathway of somites 1-5
throughout collar formation.