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Figure 10


Fig. 10. Morpholino-mediated knock-down of sdf1a generates melanophore patterning defects in 55 hpf embryos. (A-D) Fixed cho mutant embryos (B,D) and WT siblings (A,C) with (C,D) or without (A,B) sdf1a morpholino. Note the decreased numbers of melanophores in the WT lateral stripe (arrow) and the cho collar (arrowhead) after morpholino injection. Scale bar: 375 µm. (E,F) Quantitation of these defects. (E) Lateral pathway collar melanophores of cho mutant embryos. (F) Lateral stripe melanophores of WT siblings. Bar graphs show mean±s.e. melanophore counts per embryo; number of embryos is indicated at base of bar. WT and cho mutants were identified by their anterior trunk myotome phenotypes. Reduction of cho mutant collar and WT lateral stripe melanophores were both highly significant; ***, Student's t-test P<0.0001.





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