|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Somite defects in cho mutants. (A-D) Transverse
sections of 5 dpf embryos reveal defective muscle block extension in anterior
(arrowheads, A,B), but not posterior (C,D), trunk of cho mutants
(B,D) as compared with WT (A,C). cho mutants typically lack
melanophores and horizontal myoseptum; exceptionally, as here (left side), a
single melanophore may be present, but in an abnormal position. (E-H)
Sections of anterior (E,F) and posterior (G,H) trunk of 24 hpf WT (E,G) and
cho mutant (F,H) embryos labelled with F59 antibody to show slow
muscle fibres (arrows in E,F). (I-L) Lateral view of anterior (I,J;
somites 1 and 5 indicated) and posterior (K,L) trunk of 24 hpf WT (I,K) and
mutant (J,L) embryos labelled with F59 antibody; note the disorganised slow
muscle fibres and apparent holes in fibre pattern (arrow) in cho
mutant. (M,N) Transverse sections of anterior (M) and posterior (N)
trunk of 30 hpf cho mutant showing smbp RNA in situ
hybridisation. (O,P) At the 26-somite stage, WT somites (O) consist
largely of fast muscle (red; antibody EB165) under surface slow muscle (green;
BAD5), whereas cho mutants (P) displayed severely decreased fast
muscle. Scale bars: A-D,I-L, 75 µm; E-H, 25 µm; M,N, 40 µm; O,P, 25
µm.
|
