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Fig. 1. EGFR activation and Delta expression in pupal cone cells. (A)
Summary of events in the larval third instar eye disc based on Flores et al.
(Flores et al., 2000) and Tsuda
et al. (Tsuda et al., 2002).
Activation of EGFR in R cells (green) causes the derepression of
Delta. This process also requires the function of two novel nuclear
proteins (Ebi and Sno) and the proteosome complex
(Tsuda et al., 2002).
Sequential and combinatorial integration of EGFR, Notch and Lz in cone-cell
precursors (yellow) causes the expression of D-Pax2 and other genes
involved in the specification of cone-cell fate. (B,C) Wild-type
mid-pupal eye disc. Delta protein (green) is expressed in apical tips of pupal
cone cells (B). The corresponding nuclei are marked by the expression of
D-Pax2 lacZ (red, C). (D-F) MAPK activation in cone cells of a
mid-pupal eye discs. Eye discs were stained for activated MAPK (red, D) and
also for D-Pax2 lacZ (green, E). These two signals co-localize in the
pupal cone-cell nuclei (yellow, F). (G-L) Delta is transcribed in pupal
but not in larval cone cells. (G-I) In third instar eye discs,
Delta-lacZ (Dl-lacZ; green, G) is not expressed in cone
cells, which are marked with Cut (red, H). Residual lacZ expression
is in R cells (notice the lack of overlap in I). (J-L) In pupal eye discs,
Dl-lacZ (green, J) is expressed in cone cells, which are marked with
Cut (red, K). The overlap is evident in the merged panel (yellow, L).
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