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Fig. 7. Characterisation of Lhx6-positive columns of cells in the
telencephalon. (A-D) Frontal sections through the telencephalon of
48 hpf surface fish (left) and cavefish embryos (right) showing Lhx6
and corresponding GAD65 expression in the putative migratory stream.
Arrows point to the increased Lhx6 or GAD65 component in
cavefish. (E,F) Horizontal sections showing that Lhx6
(E) and Nkx2.1 (F) patterns are indistinguishable at 60 hpf in the
columns of cells. (G-I) DiI labelling of the olfactory projection onto
the olfactory bulbs of a 7-day-old cavefish, in dorsal views, where anterior
is up. G (bright field), H (fluorescence) and I (combined) photomicrographs
indicate that the olfactory bulbs are located in the anterolateral part of
Astyanax telencephalon, i.e. where the Lhx6- and
GAD65-positive cells terminate their migration. (J-M)
Hemi-sections through the telencephalon of surface (J,K) or cavefish (L,M)
embryos, to illustrate Lhx6 pattern with regard to Pax6 and
Lhx9. In L,M, dashed lines delineate the brain and the adjacent
olfactory epithelium (oe), which also expresses Lhx9. (N)
Schema, in which gene expression is indicated on the left, with corresponding
anatomical regions on the right. dSP, dorsal part of subpallium; hyp,
hypothalamus; n, nostril; ob, olfactory bulb; po, preoptic region; SP,
subpallium; tel, telencephalon. Scale bars: 50 µm.
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