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Figure 4


Fig. 4. Sema3D depletion disrupts innervation of the dorsal IPN. Larvae were stained with anti-Lov (red) and anti-acetylated tubulin (green) at 4 days. (A-H) Dorsal views of the brain in control (A), Nrp1a (B), Sema3Aa (C), Sema3Ab (D), Sema3D (E), Sema3Fa (F), Sema3Ga (G), or Sema3Gb (H) MO-injected embryos. Optimal concentrations of MOs (see Table 1) showed no effect on general brain morphology. (I-V) Dorsal views of the habenular nuclei (I-K) and lateral views of the IPN (L-V) of (I,L,O) mock-injected larvae or larvae injected with antisense MOs for (J,M,P) Sema3D, (K,N,Q) Sema3D and Nrp1a, (R) Sema3Aa, (S) Sema3Ab, (T) Sema3Fa, (U) Sema3Ga or (V) Sema3Gb. Scale bars: 50 µm.





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