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Fig. 5. Dose-dependence and synergism of Nrp1a and Sema3D MOs. Innervation
of the IPN was assayed by anti-Lov immunolabeling and confocal microscopy and
the number of larvae lacking dorsal projections (% abnormal dorsal IPN
innervation) was determined for each experimental condition. Error bars
represent the s.e.m. from at least three independent injection experiments.
For a given MO, high MO refers to the concentration that caused morphological
defects and lethality in a large proportion of larvae (above 50%); optimal MO
concentrations did not produce morphological defects or lethality above
mock-injected larvae; low MO concentrations were at least 25% less effective
than the optimal MO concentration at causing defective IPN innervation.
Co-injection of low MO amounts for Nrp1a and Sema3D had a significantly
greater effect than injection of either MO alone. For the total number of
larvae assayed and other experimental details see
Table 1.
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