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Fig. 3. Leaves are initiated from a pair of epidermal cells. (A-D)
Schematic of full-length leaf sectors that traversed all three Ad-Ab leaf
layers and support a two-cell (A), four-cell (B), six-cell (C) and eight-cell
(D) mediolaterally extended strip of cells that are recruited from the
meristem to make a leaf. (E) Schematic of sectors occupying two of the
three leaf layers (i.e. those that are formed when the Ad-Ab axis comprises
two cell layers). Sectors are a maximum of a one-sixth leaf width indicating
that the Ad-Ab axis is not established until at least six cells have been
recruited in the M-L axis. Notably, some sectors traverse the full length of
the leaf indicating that Ad-Ab axis formation precedes P-D axis formation.
(F) Schematic of sectors occupying just one cell layer (i.e. those that
are formed when the Ad-Ab axis comprises three cell layers). No sectors
traverse the full length of the leaf showing that the P-D axis is established
before divisions in the Ad-Ab axis are completed. (G) Summary of
earliest cell division patterns in the leaf as inferred from 1362 sectors. At
the six-cell stage, the next cell division can either be in the M-L axis to
form an eight-cell strip, or in the Ad-Ab axis to produce 12 founder
cells.
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