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Fig. 1. Cort and Fzy are required for the completion of meiosis I and meiosis
II. (A-L') Microtubules are red, a 359 bp centromeric repeat
unique to the X-chromosome is labeled green and DNA is blue. Smaller panels
(A'-L') are higher-magnification views of one of the spindles in
A-L, respectively. Mitotic spindles derived from the male pronucleus (when
present) are more internal and are not shown in these images. (A-C) Meiosis in
wild-type eggs obtained by in vitro activation (A) or from 0-20 minute egg
collections (B,C). In metaphase of meiosis I (A), the egg contains a single
spindle with two discrete X-chromosome signals representing two pairs of
sister chromatids. In metaphase II (B), there are two tandemly arranged
spindles, each containing a single X-chromosome signal. In anaphase II (C),
sister chromatids separate and both spindles contain two discrete X-chromosome
signals. (D-L) Eggs at 0-2 hours from females of the genotypes indicated. (D)
In cortQW55/cortRH65 (females kept at 29°C
for 3-5 days), eggs contain two spindles, each with one X-chromosome,
indicating arrest in metaphase II. (E,F)
Cks30AKO/Cks30AKO eggs with two spindles and
either one (E) or two (F) X-chromosomes, indicating a delay as early as
metaphase of meiosis II. (G) In
fzy6/fzy7 kept at 29°C, the
majority of eggs contain two spindles, both with two X-chromosome signals,
indicating arrest in anaphase II. Arrow indicates a small spindle and
associated chromatin. (H,I) In
cortQW55,fzy6/cortRH65,fzy7
double mutants kept at 29°C, eggs contain either two spindles with one
X-chromosome each (H), indicating a metaphase II arrest, or a single spindle
with two X-chromosomes (I), indicating a metaphase I arrest. (J-L) Eggs from
nosGal4VP16/UAS-cyclinB-TPM-GFP arrest with multiple spindles (J) or with two
spindles (K), suggesting a meiosis II arrest, or with a single spindle (L),
indicative of a meiosis I arrest.
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