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Figure 4


Fig. 4. Cyclin B associates with the spindle in female meiosis. Wild-type (yw) eggs obtained by in vitro activation of oocytes (A-D') or from 0-20 minute egg collections (E-G'). In all panels except B,B', microtubules are red, DNA is green and cyclin B is blue. The cyclin B channel is shown by itself in grayscale in right-hand panels (labeled with '). (A) In metaphase I, high levels of cyclin B accumulate on the spindle mid-zone. (B,B') Metaphase I spindle labeled with antibodies to Sub (B) and cyclin B (B') reveals colocalization at the spindle mid-zone. (C,D) In anaphase I, cyclin B persists at the spindle mid-zone. (E) In metaphase II, cyclin B accumulates at the mid-zone on both spindles. (F) Cyclin B persists at lower levels at the mid-zone in anaphase II. (G) Late in anaphase II, cyclin B is no longer detected on the meiotic spindle. Scale bars: 5 µm in A for A,A',C-G'; 2 µm in B for B,B'.





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